Gibson Assembly® | NEB
https://www.neb.com/en/applications/cloning-and-synthetic-biology/dna-assembly-and-cloning/gibson-assembly
WebDaniel G. Gibson, of the J. Craig Venter Institute, described a robust exonuclease-based method to assemble DNA seamlessly and in the correct order, eponymously known as Gibson Assembly. The reaction is carried out under isothermal conditions using three enzymatic activities: a 5’ exonuclease generates long overhangs, a polymerase fills in ...
DA: 29 PA: 13 MOZ Rank: 84