Keyword Analysis & Research: t7 promoter


Keyword Analysis

Keyword CPC PCC Volume Score Length of keyword
t7 promoter1.040.862993011
t70.8213563652
promoter0.030.77248778

Keyword Research: People who searched t7 promoter also searched

Keyword CPC PCC Volume Score
t7 promoter1.480.6541521

Search Results related to t7 promoter on Search Engine

  • T7 expression system - Wikipedia

    wikipedia.org

    https://en.wikipedia.org/wiki/T7_expression_system

    WebMechanism. An expression vector, most commonly the pET expression vector, is engineered to integrate two essential components: a T7 promoter and a gene of interest downstream of the promoter and under its control. The expression vector is transformed into one of several relevant strains of E. coli, most frequently BL21 (DE3).

    DA: 24 PA: 8 MOZ Rank: 71

  • T7 RNA polymerase - Wikipedia

    wikipedia.org

    https://en.wikipedia.org/wiki/T7_RNA_polymerase

    WebT7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a double stranded DNA template and Mg 2+ ion as cofactor for the synthesis of RNA. It has a very low error rate. T7 polymerase has a molecular weight of 99 kDa. Promoter

    DA: 80 PA: 94 MOZ Rank: 18

  • Maximizing transcription of nucleic acids with efficient T7 promoters …

    nature.com

    https://www.nature.com/articles/s42003-020-01167-x

    WebAug 14, 2020 · 26 Citations. 39 Altmetric. Metrics. In vitro transcription using T7 bacteriophage polymerase is widely used in molecular biology. Here, we use 5′RACE-Seq to screen a randomized initially...

    DA: 77 PA: 13 MOZ Rank: 88

  • An engineered T7 RNA polymerase that produces mRNA free of

    nature.com

    https://www.nature.com/articles/s41587-022-01525-6

    WebNov 10, 2022 · For many research and commercial applications, IVT of mRNA is typically performed using bacteriophage T7 RNA polymerase (T7 RNAP) owing to its ability to produce full-length RNA transcripts...

    DA: 74 PA: 56 MOZ Rank: 45

  • T7Max transcription system - PMC - National Center for …

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9872363/

    WebJan 23, 2023 · Results. Here we present characterization of a variant of T7 RNA polymerase promoter that acts to significantly increase the yields of gene expression within in vitro systems. We have demonstrated that T7Max increases the yield of translation in many types of commonly used in vitro protein expression systems.

    DA: 9 PA: 35 MOZ Rank: 67


  • A specific, promoter-independent activity of T7 RNA …

    nature.com

    https://www.nature.com/articles/s41598-018-32231-6

    WebSep 17, 2018 · A specific, promoter-independent activity of T7 RNA polymerase suggests a general model for DNA/RNA editing in single subunit RNA Polymerases | Scientific Reports. Article. Open access....

    DA: 25 PA: 81 MOZ Rank: 88

  • Maximizing transcription of nucleic acids with efficient T7 promoters

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429497/

    WebAug 14, 2020 · We reveal that sequences from position +4 to +8 downstream of the transcription start site affect T7 promoter activity over a 5-fold range, and identify promoter variants with significantly enhanced transcriptional output that increase the yield of in vitro transcription reactions across a wide range of template concentrations.

    DA: 84 PA: 30 MOZ Rank: 22

  • Regulating the T7 RNA polymerase expression in

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474846/

    WebSep 26, 2021 · The T7 RNAP specifically recognizes the T7 promoter and transcribes eight times faster than E. coli RNAP [2–4]. The gene encoding the T7 RNAP is governed by the isopropyl β-d-1-thiogalactopyranoside (IPTG)-inducible lacUV5 promoter (P lacUV5), which is a strong variant of the wild-type lac promoter (P lacWT) . The reasoning behind the choice ...

    DA: 72 PA: 30 MOZ Rank: 69

  • Promoter specificity determinants of T7 RNA polymerase | PNAS

    pnas.org

    https://www.pnas.org/doi/full/10.1073/pnas.95.2.515

    WebJan 20, 1998 · During the early stages of transcription, T7 RNAP engages in repeated cycles of abortive initiation in which short RNA products are synthesized and released before the polymerase clears the promoter and forms a stable elongation complex ( 12 – 14 ).

    DA: 33 PA: 43 MOZ Rank: 8

  • T7 Promoter System - MilliporeSigma

    sigmaaldrich.com

    https://www.sigmaaldrich.com/SE/en/technical-documents/technical-article/genomics/cloning-and-expression/t7-promoter-system

    WebThe T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase 1 . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications 2.

    DA: 11 PA: 17 MOZ Rank: 2