t7 promoter sequence | T7 promoter Sequence and Map

Keyword	CPC	PCC	Volume	Score	Length of keyword
t7 promoter sequence	1.43	0.4	9596	66	20
t7	0.22	0.5	7601	6	2
promoter	0.84	0.2	2855	11	8
sequence	1.69	1	1395	81	8

Keyword	CPC	PCC	Volume	Score
t7 promoter sequence	1.34	0.2	7890	95
t7 promoter sequence design	1.39	0.1	2896	16
t7 promoter sequence cloning	1.08	0.4	1397	61
t7 promoter sequence database	1.41	0.4	9858	72
t7 promoter sequence mutation	1.77	0.1	4178	61
t7 promoter sequence alignment	0.3	0.8	7139	57
t7 promoter sequence variation	1.15	0.9	9086	33
t7 promoter sequence comparison	0.47	0.1	7733	58
t7 promoter sequence expression	0.05	0.4	2942	77
t7 promoter sequence regulation	0.22	1	7929	25
t7 promoter sequence optimization	0.19	0.2	8944	1
t7 promoter sequence neb	1.2	0.2	7182	63
t7 promoter sequence primer	1.09	0.6	4095	87
t7 promoter sequence transcription start site	0.05	0.8	514	21
t7 promoter sequence start of transcription	1.54	0.1	930	18
t7 rna polymerase promoter sequence	0.45	0.6	8001	11

Search Results related to t7 promoter sequence on Search Engine

T7 promoter Sequence and Map - SnapGene
snapgene.com

https://www.snapgene.com/plasmids/basic_cloning_vectors/T7_promoter

WEBT7 promoter Sequence and Map. Promoter for bacteriophage T7 RNA polymerase. Open in SnapGene. Try SnapGene for Free. Download Plasmid. | Download SnapGene Viewer. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Home Plasmids Basic Cloning VectorsT7 promoter. Show Static Map.

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Maximizing transcription of nucleic acids with efficient T7 promoters
nature.com

https://www.nature.com/articles/s42003-020-01167-x

WEBAug 14, 2020 · We reveal that sequences from position +4 to +8 downstream of the transcription start site affect T7 promoter activity over a 5-fold range, and identify promoter variants with significantly...

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What is the promoter sequence of T7 RNA Polymerase? | NEB
neb.com

https://www.neb.com/en/faqs/2015/01/30/what-is-the-promoter-sequence-of-t7-rna-polymerase

WEBJan 30, 2015 · FAQ: What is the promoter sequence of T7 RNA Polymerase? T7 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from 5’->3’. The first base in the transcript will be a G.

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T7 Promoter System - MilliporeSigma
sigmaaldrich.com

https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/cloning-and-expression/t7-promoter-system

WEBWhat Is the T7 Promoter Sequence? The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase 1. The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of ...

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T7 expression system - Wikipedia
wikipedia.org

https://en.wikipedia.org/wiki/T7_expression_system

WEBT7 RNA polymerase is responsible for beginning transcription at the T7 promoter of the transformed vector. The T7 gene is itself under the control of a lac promoter. Normally, both the lac promoter and the T7 promoter are repressed in …

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T7 RNA polymerase - Wikipedia
wikipedia.org

https://en.wikipedia.org/wiki/T7_RNA_polymerase

WEBT7 polymerase is extremely promoter -specific and transcribes only DNA downstream of a T7 promoter. [2] The T7 polymerase also requires a double stranded DNA template and Mg 2+ ion as cofactor for the synthesis of RNA. It has a very low error rate. T7 polymerase has a molecular weight of 99 kDa.

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T7 RNA Polymerase - an overview | ScienceDirect Topics
sciencedirect.com

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/t7-rna-polymerase

WEBT7 RNA polymerase (T7 RNAP) requires the presence of a T7 promoter sequence (TAATACGACTCACTATA) followed by at least one guanosine (Imburgio, Rong, Ma, & McAllister, 2000). We recommend appending 2 or 3 guanosine nucleotides after the T7 promoter sequence to achieve the best efficiency of T7 transcription.

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Promoter specificity determinants of T7 RNA polymerase | PNAS
pnas.org

https://www.pnas.org/doi/full/10.1073/pnas.95.2.515

WEBJan 20, 1998 · To identify potential contacts between amino acids in the specificity loop and bp in the upstream region of the promoter, we compared the DNA sequences of the T7, T3, SP6, and K11 promoters (Fig. 1) and the amino acid sequences of these RNAPs in the region comprising the specificity loop (Fig. 2 ).

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Mechanism and Structure – Martin Lab - UMass
umass.edu

https://martinlab.chem.umass.edu/about-t7-rna-polymerase/

WEBThe consensus promoter sequence is derived from the 17 known promoters in the bacteriophage T7 genome. Class III promoters are generally considered to be the strongest promoters and are the most highly conserved. Thus, the consensus promoter sequence is generally considered to be. TAATACGACTCACTATAGGG. and extends from position …

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T7Max transcription system - PMC - National Center for …
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9872363/

WEBJan 23, 2023 · The T7 promoter for the RNA polymerase of bacteriophage T7 consists of 18 base pairs of sequence (5’ – TAATACGACTCACTATAG – 3’ ) [ 1 ]. Previous research identified the relationship between the sequence and transcriptional efficiency, which helped to strengthen the T7 system’s usability [ 2 – 4 ].

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