bicistronic expression | Plasmids 101: Multicistronic Vectors

Keyword	CPC	PCC	Volume	Score	Length of keyword
bicistronic expression	0.55	0.3	1464	92	22
bicistronic	0.33	0.6	3565	73	11
expression	1.95	0.7	9506	100	10

Keyword	CPC	PCC	Volume	Score
bicistronic expression	1.51	0.1	6420	3
bicistronic expression vector	1.44	0.2	2793	88
bicistronic expression system	0.67	0.7	6802	66
ires bicistronic expression vector	0.14	0.5	4459	84
bicistronic mammalian expression vector	1.36	0.3	4135	38

Search Results related to bicistronic expression on Search Engine

Plasmids 101: Multicistronic Vectors - Addgene
addgene.org

https://blog.addgene.org/plasmids-101-multicistronic-vectors

WEBAddgene's collection offers a variety of plasmids to express two or more genes, some of which are listed below. We should note that t hese vectors are primarily designed for bicistronic expression; however, many could be easily manipulated to express more than two genes. Plasmid Name. Multicistronic Element.

DA: 10 PA: 28 MOZ Rank: 24

Development of bicistronic expression system for the enhanced …
nature.com

https://www.nature.com/articles/s41598-018-27091-z

WEBJun 11, 2018 · In this study, for the enhanced and reliable production of recombinant proteins in L. citreum, we developed a bicistronic design (BCD) expression system which includes a short leader peptide (1...

DA: 61 PA: 62 MOZ Rank: 73

Bicistronic expression strategy for high‐level expression of
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999366/

WEBAug 14, 2017 · A bicistronic expression part containing the short 5′ coding sequence of the source gene and an embedded Shine–Dalgarno sequence can cause higher expression levels of the recombinant gene in a bicistronic cassette.

DA: 56 PA: 81 MOZ Rank: 37

Bicistronic design as recombinant expression enhancer ... - Springer
springer.com

https://link.springer.com/article/10.1007/s00253-021-11611-5

WEBOct 1, 2021 · Sun, M., Gao, A.X., Li, A. et al. Bicistronic design as recombinant expression enhancer: characteristics, applications, and structural optimization. Appl Microbiol Biotechnol 105, 7709–7720 (2021). https://doi.org/10.1007/s00253-021-11611-5. Download citation. Received: 20 July 2021. Revised: 16 September 2021. Accepted: 18 …

DA: 79 PA: 99 MOZ Rank: 30

Bicistronic Design-Based Continuous and High-Level Membrane …
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646956/

WEBJul 7, 2019 · The current methods to produce membrane proteins in this organism require the induction of target gene expression and often result in unstable, low yields. Here, we present a method combining a constitutive promoter with a library of bicistronic design (BCD) elements, which enables inducer-free, tuned translation initiation for optimal …

DA: 89 PA: 88 MOZ Rank: 54

Express yourself: Gene Co-Expression in Bicistronic Constructs
bitesizebio.com

https://bitesizebio.com/24492/express-yourself-gene-co-expression-in-bicistronic-constructs/

WEBJun 15, 2015 · To sum up, choosing the right method for co-expression depends on your specific needs and limitations. The two-promoter system is powerful and efficient as long as you have enough space within your vector. IRES is a useful alternative when space is limited and the expression ratio is not important.

DA: 70 PA: 28 MOZ Rank: 73

Current Practice in Bicistronic IRES Reporter Use: A ... - PubMed
nih.gov

https://pubmed.ncbi.nlm.nih.gov/34068921/

WEBPMID: 34068921. PMCID: PMC8156625. DOI: 10.3390/ijms22105193. Abstract. Bicistronic reporter assays have been instrumental for transgene expression, understanding of internal ribosomal entry site (IRES) translation, and identification of novel cap-independent translational elements (CITE).

DA: 63 PA: 98 MOZ Rank: 71

Bicistronic design as recombinant expression enhancer: …
nih.gov

https://pubmed.ncbi.nlm.nih.gov/34596722/

WEBThe bicistronic design (BCD) is characterized by a short fore-cistron sequence and a second Shine-Dalgarno (SD2) sequence upstream of the target gene. The outstanding performance of this expression cassette in promoting recombinant protein production has attracted attention.

DA: 61 PA: 86 MOZ Rank: 59

Bicistronic design as recombinant expression enhancer
springer.com

https://link.springer.com/content/pdf/10.1007/s00253-021-11611-5.pdf

WEBThe bicistronic expression cassette is derived from the polycistron, a common genetic structure in archaea and bacteria (Schümperli et al. 1982; Huber et al. 2019). In a polycistron, two or more gene sequences are controlled by a single promoter (Fig. 1A), which enables the synthesis of polycistronic mRNA.

DA: 8 PA: 84 MOZ Rank: 75

Concerted expression of a cell cycle regulator and a metabolic
nature.com

https://www.nature.com/articles/s41477-019-0358-3

WEBFeb 8, 2019 · 20 Citations. 10 Altmetric. Metrics. Abstract. Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF)....

DA: 17 PA: 73 MOZ Rank: 96