Double FYVE-containing protein 1 (DFCP1) encodes a 777 amino acid protein that contains: (1) an N-terminal Cys–His cluster with some homology to many zinc finger domains; (2) a consensus sequence consistent with an ATP/GTP binding site; and (3) a C-terminal domain unique because it contains two zinc-binding FYVE domains.Does the ER-localized protein dfcp1 modulate ER-lipid droplet contact formation?
The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation Very little is known about the spatiotemporal generation of lipid droplets (LDs) from the endoplasmic reticulum (ER) and the factors that mediate ER-LD contacts for LD growth.How similar are the FYVE domains in dfcp1?
In DFCP1, both FYVE domains are approximately 70 amino acids long (solid lined box, Fig. 1A) and share 53% amino acid identity and 56% similarity ( Fig. 2A, first two rows). The two FYVE domains are approximately 40% identical and 53% similar to FYVE domains of other proteins ( Fig. 2A ).Does the FYVE-domain-containing ER-localized protein dfcp1 migrate to omegasomes during autophagy?
The FYVE-domain-containing ER-localized protein DFCP1 (also known as ZFYVE1) migrates to omegasomes upon autophagy induction, a process that depends on both the ER localization domain and the PI (3)P-binding FYVE domain ( Axe et al., 2008 ).